total pkr (Santa Cruz Biotechnology)
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total pkr
Total Pkr, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 93/100, based on 49 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/total+pkr/PKR/pmc10480325-15-0-3
Average 93 stars, based on 49 article reviews
Total Pkr, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 93/100, based on 49 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/total+pkr/PKR/pmc10480325-15-0-3
Average 93 stars, based on 49 article reviews
total pkr - by Bioz Stars,
2026-09
93/100 stars
Images
1) Product Images from "Viral sensing by epithelial cells involves PKR- and caspase-3-dependent generation of gasdermin E pores"
Article Title: Viral sensing by epithelial cells involves PKR- and caspase-3-dependent generation of gasdermin E pores
Journal: iScience
doi: 10.1016/j.isci.2023.107698
Figure Legend Snippet: DsRNA-stimulated GSDME pore formation, GSDMD inactivation, and caspase-3 activation are PKR dependent NHBE cells were treated with DMSO vehicle control or PKR inhibitor C16 (2 μM) for 1 h before transfection with 2.5 μg/mL pIC. (A) Inhibition of dsRNA-stimulated phosphorylation of PKR by C16 treatment. Cell lysates (WCL) were harvested at 6 h after treatment and immunoblotted for phospho-PKR ( p -PKR), total PKR, and β-actin. (B) GSDME activation and GSDMD inactivation are PKR dependent. Cell lysates (WCL) and supernatants (SN) were harvested at 6 h and immunoblotted for GSDMD, GSDME, caspase-3, and β-actin. (C) Lytic cell death was assessed by measuring LDH release in the supernatant at 8 and 24 h. (D–F) Secretion of IL-1β (D), IL-1α (E), and IL-6 (F) was quantified by ELISA at 8 and 24 h. Immunoblots are representative of three independent experiments. Other data are mean ± SEM of three independent biological experiments, each performed in triplicate. ns: not significant, ∗p < 0.05, ∗∗p < 0.01 and ∗∗∗p < 0.001 by unpaired Student’s t test.
Techniques Used: Activation Assay, Transfection, Inhibition, Enzyme-linked Immunosorbent Assay, Western Blot
Figure Legend Snippet:
Techniques Used: Virus, Recombinant, Molecular Weight, Enzyme-linked Immunosorbent Assay, Cytotoxicity Assay, Lactate Dehydrogenase Assay, Sequencing, Negative Control, Software
Related Articles
Activation Assay:Article Title: nc886 is induced by TGF-β and suppresses the microRNA pathway in ovarian cancer Article Snippet: The information about antibodies is; phospho-PKR (cat# ab32036 from AbCam, Cambridge, MA; diluted to 1/3000), total PKR (cat# sc-707 from Santa Cruz Biotechnology, Dallas, TX; diluted to 1/3000), phospho-eIF2α (cat# 9721S from Cell Signaling Technology, Danvers, MA; diluted to 1/3000), total eIF2α (cat# 9722S from Cell Signaling Technology; diluted to 1/3000), and Article Title: Mechanism of Protein Kinase R Inhibition by Human Cytomegalovirus pTRS1 Article Snippet: Antibodies against the following were used: pTRS1 (1:100 [ 43 ]), His (1:1,000; CST; number 2365S), total Article Title: Crystal structure of an adenovirus virus-associated RNA Article Snippet: Additionally, total PKR was quantified on a separate slot blot using Article Title: Viral sensing by epithelial cells involves PKR- and caspase-3-dependent generation of gasdermin E pores Article Snippet: Total PKR , Article Title: Viral sensing by epithelial cells involves PKR- and caspase-3-dependent generation of gasdermin E pores Article Snippet: Nitrocellulose membranes were probed with primary antibodies for either GSDMD (G7422, Sigma-Aldrich 1/1000; HPA044487, Sigma-Aldrich, 1/1000), GSDME (ab215191, Abcam, 1/1000), Caspase-1 (AG-20B-0048, Adipogen, 1/1000), Caspase-3 (9661, Cell signaling, 1/1000), Caspase-8 (9746, Cell Signaling, 1/500), IL-1α (500-P21A, PeproTech, 1/500), IL-1β (AF-201-NA, R&D, 1/1000), Article Title: Crystal structure of an adenovirus virus-associated RNA. Article Snippet: Additionally, total PKR was quantified on a separate slot blot using Transfection:Article Title: nc886 is induced by TGF-β and suppresses the microRNA pathway in ovarian cancer Article Snippet: The information about antibodies is; phospho-PKR (cat# ab32036 from AbCam, Cambridge, MA; diluted to 1/3000), total PKR (cat# sc-707 from Santa Cruz Biotechnology, Dallas, TX; diluted to 1/3000), phospho-eIF2α (cat# 9721S from Cell Signaling Technology, Danvers, MA; diluted to 1/3000), total eIF2α (cat# 9722S from Cell Signaling Technology; diluted to 1/3000), and Article Title: Mechanism of Protein Kinase R Inhibition by Human Cytomegalovirus pTRS1 Article Snippet: Antibodies against the following were used: pTRS1 (1:100 [ 43 ]), His (1:1,000; CST; number 2365S), total Article Title: Crystal structure of an adenovirus virus-associated RNA Article Snippet: Additionally, total PKR was quantified on a separate slot blot using Article Title: Viral sensing by epithelial cells involves PKR- and caspase-3-dependent generation of gasdermin E pores Article Snippet: Total PKR , Article Title: Viral sensing by epithelial cells involves PKR- and caspase-3-dependent generation of gasdermin E pores Article Snippet: Nitrocellulose membranes were probed with primary antibodies for either GSDMD (G7422, Sigma-Aldrich 1/1000; HPA044487, Sigma-Aldrich, 1/1000), GSDME (ab215191, Abcam, 1/1000), Caspase-1 (AG-20B-0048, Adipogen, 1/1000), Caspase-3 (9661, Cell signaling, 1/1000), Caspase-8 (9746, Cell Signaling, 1/500), IL-1α (500-P21A, PeproTech, 1/500), IL-1β (AF-201-NA, R&D, 1/1000), Article Title: Crystal structure of an adenovirus virus-associated RNA. Article Snippet: Additionally, total PKR was quantified on a separate slot blot using Inhibition:Article Title: nc886 is induced by TGF-β and suppresses the microRNA pathway in ovarian cancer Article Snippet: The information about antibodies is; phospho-PKR (cat# ab32036 from AbCam, Cambridge, MA; diluted to 1/3000), total PKR (cat# sc-707 from Santa Cruz Biotechnology, Dallas, TX; diluted to 1/3000), phospho-eIF2α (cat# 9721S from Cell Signaling Technology, Danvers, MA; diluted to 1/3000), total eIF2α (cat# 9722S from Cell Signaling Technology; diluted to 1/3000), and Article Title: Mechanism of Protein Kinase R Inhibition by Human Cytomegalovirus pTRS1 Article Snippet: Antibodies against the following were used: pTRS1 (1:100 [ 43 ]), His (1:1,000; CST; number 2365S), total Article Title: Crystal structure of an adenovirus virus-associated RNA Article Snippet: Additionally, total PKR was quantified on a separate slot blot using Article Title: Viral sensing by epithelial cells involves PKR- and caspase-3-dependent generation of gasdermin E pores Article Snippet: Total PKR , Article Title: Viral sensing by epithelial cells involves PKR- and caspase-3-dependent generation of gasdermin E pores Article Snippet: Nitrocellulose membranes were probed with primary antibodies for either GSDMD (G7422, Sigma-Aldrich 1/1000; HPA044487, Sigma-Aldrich, 1/1000), GSDME (ab215191, Abcam, 1/1000), Caspase-1 (AG-20B-0048, Adipogen, 1/1000), Caspase-3 (9661, Cell signaling, 1/1000), Caspase-8 (9746, Cell Signaling, 1/500), IL-1α (500-P21A, PeproTech, 1/500), IL-1β (AF-201-NA, R&D, 1/1000), Article Title: Crystal structure of an adenovirus virus-associated RNA. Article Snippet: Additionally, total PKR was quantified on a separate slot blot using Enzyme-linked Immunosorbent Assay:Article Title: nc886 is induced by TGF-β and suppresses the microRNA pathway in ovarian cancer Article Snippet: The information about antibodies is; phospho-PKR (cat# ab32036 from AbCam, Cambridge, MA; diluted to 1/3000), total PKR (cat# sc-707 from Santa Cruz Biotechnology, Dallas, TX; diluted to 1/3000), phospho-eIF2α (cat# 9721S from Cell Signaling Technology, Danvers, MA; diluted to 1/3000), total eIF2α (cat# 9722S from Cell Signaling Technology; diluted to 1/3000), and Article Title: Mechanism of Protein Kinase R Inhibition by Human Cytomegalovirus pTRS1 Article Snippet: Antibodies against the following were used: pTRS1 (1:100 [ 43 ]), His (1:1,000; CST; number 2365S), total Article Title: Crystal structure of an adenovirus virus-associated RNA Article Snippet: Additionally, total PKR was quantified on a separate slot blot using Article Title: Viral sensing by epithelial cells involves PKR- and caspase-3-dependent generation of gasdermin E pores Article Snippet: Total PKR , Article Title: Viral sensing by epithelial cells involves PKR- and caspase-3-dependent generation of gasdermin E pores Article Snippet: Nitrocellulose membranes were probed with primary antibodies for either GSDMD (G7422, Sigma-Aldrich 1/1000; HPA044487, Sigma-Aldrich, 1/1000), GSDME (ab215191, Abcam, 1/1000), Caspase-1 (AG-20B-0048, Adipogen, 1/1000), Caspase-3 (9661, Cell signaling, 1/1000), Caspase-8 (9746, Cell Signaling, 1/500), IL-1α (500-P21A, PeproTech, 1/500), IL-1β (AF-201-NA, R&D, 1/1000), Article Title: Crystal structure of an adenovirus virus-associated RNA. Article Snippet: Additionally, total PKR was quantified on a separate slot blot using Western Blot:Article Title: nc886 is induced by TGF-β and suppresses the microRNA pathway in ovarian cancer Article Snippet: The information about antibodies is; phospho-PKR (cat# ab32036 from AbCam, Cambridge, MA; diluted to 1/3000), total PKR (cat# sc-707 from Santa Cruz Biotechnology, Dallas, TX; diluted to 1/3000), phospho-eIF2α (cat# 9721S from Cell Signaling Technology, Danvers, MA; diluted to 1/3000), total eIF2α (cat# 9722S from Cell Signaling Technology; diluted to 1/3000), and Article Title: Mechanism of Protein Kinase R Inhibition by Human Cytomegalovirus pTRS1 Article Snippet: Antibodies against the following were used: pTRS1 (1:100 [ 43 ]), His (1:1,000; CST; number 2365S), total Article Title: Crystal structure of an adenovirus virus-associated RNA Article Snippet: Additionally, total PKR was quantified on a separate slot blot using Article Title: Viral sensing by epithelial cells involves PKR- and caspase-3-dependent generation of gasdermin E pores Article Snippet: Total PKR , Article Title: Viral sensing by epithelial cells involves PKR- and caspase-3-dependent generation of gasdermin E pores Article Snippet: Nitrocellulose membranes were probed with primary antibodies for either GSDMD (G7422, Sigma-Aldrich 1/1000; HPA044487, Sigma-Aldrich, 1/1000), GSDME (ab215191, Abcam, 1/1000), Caspase-1 (AG-20B-0048, Adipogen, 1/1000), Caspase-3 (9661, Cell signaling, 1/1000), Caspase-8 (9746, Cell Signaling, 1/500), IL-1α (500-P21A, PeproTech, 1/500), IL-1β (AF-201-NA, R&D, 1/1000), Article Title: Crystal structure of an adenovirus virus-associated RNA. Article Snippet: Additionally, total PKR was quantified on a separate slot blot using Virus:Article Title: nc886 is induced by TGF-β and suppresses the microRNA pathway in ovarian cancer Article Snippet: The information about antibodies is; phospho-PKR (cat# ab32036 from AbCam, Cambridge, MA; diluted to 1/3000), total PKR (cat# sc-707 from Santa Cruz Biotechnology, Dallas, TX; diluted to 1/3000), phospho-eIF2α (cat# 9721S from Cell Signaling Technology, Danvers, MA; diluted to 1/3000), total eIF2α (cat# 9722S from Cell Signaling Technology; diluted to 1/3000), and Article Title: Mechanism of Protein Kinase R Inhibition by Human Cytomegalovirus pTRS1 Article Snippet: Antibodies against the following were used: pTRS1 (1:100 [ 43 ]), His (1:1,000; CST; number 2365S), total Article Title: Crystal structure of an adenovirus virus-associated RNA Article Snippet: Additionally, total PKR was quantified on a separate slot blot using Article Title: Viral sensing by epithelial cells involves PKR- and caspase-3-dependent generation of gasdermin E pores Article Snippet: Total PKR , Article Title: Viral sensing by epithelial cells involves PKR- and caspase-3-dependent generation of gasdermin E pores Article Snippet: Nitrocellulose membranes were probed with primary antibodies for either GSDMD (G7422, Sigma-Aldrich 1/1000; HPA044487, Sigma-Aldrich, 1/1000), GSDME (ab215191, Abcam, 1/1000), Caspase-1 (AG-20B-0048, Adipogen, 1/1000), Caspase-3 (9661, Cell signaling, 1/1000), Caspase-8 (9746, Cell Signaling, 1/500), IL-1α (500-P21A, PeproTech, 1/500), IL-1β (AF-201-NA, R&D, 1/1000), Article Title: Crystal structure of an adenovirus virus-associated RNA. Article Snippet: Additionally, total PKR was quantified on a separate slot blot using Recombinant:Article Title: nc886 is induced by TGF-β and suppresses the microRNA pathway in ovarian cancer Article Snippet: The information about antibodies is; phospho-PKR (cat# ab32036 from AbCam, Cambridge, MA; diluted to 1/3000), total PKR (cat# sc-707 from Santa Cruz Biotechnology, Dallas, TX; diluted to 1/3000), phospho-eIF2α (cat# 9721S from Cell Signaling Technology, Danvers, MA; diluted to 1/3000), total eIF2α (cat# 9722S from Cell Signaling Technology; diluted to 1/3000), and Article Title: Mechanism of Protein Kinase R Inhibition by Human Cytomegalovirus pTRS1 Article Snippet: Antibodies against the following were used: pTRS1 (1:100 [ 43 ]), His (1:1,000; CST; number 2365S), total Article Title: Crystal structure of an adenovirus virus-associated RNA Article Snippet: Additionally, total PKR was quantified on a separate slot blot using Article Title: Viral sensing by epithelial cells involves PKR- and caspase-3-dependent generation of gasdermin E pores Article Snippet: Total PKR , Article Title: Viral sensing by epithelial cells involves PKR- and caspase-3-dependent generation of gasdermin E pores Article Snippet: Nitrocellulose membranes were probed with primary antibodies for either GSDMD (G7422, Sigma-Aldrich 1/1000; HPA044487, Sigma-Aldrich, 1/1000), GSDME (ab215191, Abcam, 1/1000), Caspase-1 (AG-20B-0048, Adipogen, 1/1000), Caspase-3 (9661, Cell signaling, 1/1000), Caspase-8 (9746, Cell Signaling, 1/500), IL-1α (500-P21A, PeproTech, 1/500), IL-1β (AF-201-NA, R&D, 1/1000), Article Title: Crystal structure of an adenovirus virus-associated RNA. Article Snippet: Additionally, total PKR was quantified on a separate slot blot using Molecular Weight:Article Title: nc886 is induced by TGF-β and suppresses the microRNA pathway in ovarian cancer Article Snippet: The information about antibodies is; phospho-PKR (cat# ab32036 from AbCam, Cambridge, MA; diluted to 1/3000), total PKR (cat# sc-707 from Santa Cruz Biotechnology, Dallas, TX; diluted to 1/3000), phospho-eIF2α (cat# 9721S from Cell Signaling Technology, Danvers, MA; diluted to 1/3000), total eIF2α (cat# 9722S from Cell Signaling Technology; diluted to 1/3000), and Article Title: Mechanism of Protein Kinase R Inhibition by Human Cytomegalovirus pTRS1 Article Snippet: Antibodies against the following were used: pTRS1 (1:100 [ 43 ]), His (1:1,000; CST; number 2365S), total Article Title: Crystal structure of an adenovirus virus-associated RNA Article Snippet: Additionally, total PKR was quantified on a separate slot blot using Article Title: Viral sensing by epithelial cells involves PKR- and caspase-3-dependent generation of gasdermin E pores Article Snippet: Total PKR , Article Title: Viral sensing by epithelial cells involves PKR- and caspase-3-dependent generation of gasdermin E pores Article Snippet: Nitrocellulose membranes were probed with primary antibodies for either GSDMD (G7422, Sigma-Aldrich 1/1000; HPA044487, Sigma-Aldrich, 1/1000), GSDME (ab215191, Abcam, 1/1000), Caspase-1 (AG-20B-0048, Adipogen, 1/1000), Caspase-3 (9661, Cell signaling, 1/1000), Caspase-8 (9746, Cell Signaling, 1/500), IL-1α (500-P21A, PeproTech, 1/500), IL-1β (AF-201-NA, R&D, 1/1000), Article Title: Crystal structure of an adenovirus virus-associated RNA. Article Snippet: Additionally, total PKR was quantified on a separate slot blot using Cytotoxicity Assay:Article Title: nc886 is induced by TGF-β and suppresses the microRNA pathway in ovarian cancer Article Snippet: The information about antibodies is; phospho-PKR (cat# ab32036 from AbCam, Cambridge, MA; diluted to 1/3000), total PKR (cat# sc-707 from Santa Cruz Biotechnology, Dallas, TX; diluted to 1/3000), phospho-eIF2α (cat# 9721S from Cell Signaling Technology, Danvers, MA; diluted to 1/3000), total eIF2α (cat# 9722S from Cell Signaling Technology; diluted to 1/3000), and Article Title: Mechanism of Protein Kinase R Inhibition by Human Cytomegalovirus pTRS1 Article Snippet: Antibodies against the following were used: pTRS1 (1:100 [ 43 ]), His (1:1,000; CST; number 2365S), total Article Title: Crystal structure of an adenovirus virus-associated RNA Article Snippet: Additionally, total PKR was quantified on a separate slot blot using Article Title: Viral sensing by epithelial cells involves PKR- and caspase-3-dependent generation of gasdermin E pores Article Snippet: Total PKR , Article Title: Viral sensing by epithelial cells involves PKR- and caspase-3-dependent generation of gasdermin E pores Article Snippet: Nitrocellulose membranes were probed with primary antibodies for either GSDMD (G7422, Sigma-Aldrich 1/1000; HPA044487, Sigma-Aldrich, 1/1000), GSDME (ab215191, Abcam, 1/1000), Caspase-1 (AG-20B-0048, Adipogen, 1/1000), Caspase-3 (9661, Cell signaling, 1/1000), Caspase-8 (9746, Cell Signaling, 1/500), IL-1α (500-P21A, PeproTech, 1/500), IL-1β (AF-201-NA, R&D, 1/1000), Article Title: Crystal structure of an adenovirus virus-associated RNA. Article Snippet: Additionally, total PKR was quantified on a separate slot blot using Lactate Dehydrogenase Assay:Article Title: nc886 is induced by TGF-β and suppresses the microRNA pathway in ovarian cancer Article Snippet: The information about antibodies is; phospho-PKR (cat# ab32036 from AbCam, Cambridge, MA; diluted to 1/3000), total PKR (cat# sc-707 from Santa Cruz Biotechnology, Dallas, TX; diluted to 1/3000), phospho-eIF2α (cat# 9721S from Cell Signaling Technology, Danvers, MA; diluted to 1/3000), total eIF2α (cat# 9722S from Cell Signaling Technology; diluted to 1/3000), and Article Title: Mechanism of Protein Kinase R Inhibition by Human Cytomegalovirus pTRS1 Article Snippet: Antibodies against the following were used: pTRS1 (1:100 [ 43 ]), His (1:1,000; CST; number 2365S), total Article Title: Crystal structure of an adenovirus virus-associated RNA Article Snippet: Additionally, total PKR was quantified on a separate slot blot using Article Title: Viral sensing by epithelial cells involves PKR- and caspase-3-dependent generation of gasdermin E pores Article Snippet: Total PKR , Article Title: Viral sensing by epithelial cells involves PKR- and caspase-3-dependent generation of gasdermin E pores Article Snippet: Nitrocellulose membranes were probed with primary antibodies for either GSDMD (G7422, Sigma-Aldrich 1/1000; HPA044487, Sigma-Aldrich, 1/1000), GSDME (ab215191, Abcam, 1/1000), Caspase-1 (AG-20B-0048, Adipogen, 1/1000), Caspase-3 (9661, Cell signaling, 1/1000), Caspase-8 (9746, Cell Signaling, 1/500), IL-1α (500-P21A, PeproTech, 1/500), IL-1β (AF-201-NA, R&D, 1/1000), Article Title: Crystal structure of an adenovirus virus-associated RNA. Article Snippet: Additionally, total PKR was quantified on a separate slot blot using Sequencing:Article Title: nc886 is induced by TGF-β and suppresses the microRNA pathway in ovarian cancer Article Snippet: The information about antibodies is; phospho-PKR (cat# ab32036 from AbCam, Cambridge, MA; diluted to 1/3000), total PKR (cat# sc-707 from Santa Cruz Biotechnology, Dallas, TX; diluted to 1/3000), phospho-eIF2α (cat# 9721S from Cell Signaling Technology, Danvers, MA; diluted to 1/3000), total eIF2α (cat# 9722S from Cell Signaling Technology; diluted to 1/3000), and Article Title: Mechanism of Protein Kinase R Inhibition by Human Cytomegalovirus pTRS1 Article Snippet: Antibodies against the following were used: pTRS1 (1:100 [ 43 ]), His (1:1,000; CST; number 2365S), total Article Title: Crystal structure of an adenovirus virus-associated RNA Article Snippet: Additionally, total PKR was quantified on a separate slot blot using Article Title: Viral sensing by epithelial cells involves PKR- and caspase-3-dependent generation of gasdermin E pores Article Snippet: Total PKR , Article Title: Viral sensing by epithelial cells involves PKR- and caspase-3-dependent generation of gasdermin E pores Article Snippet: Nitrocellulose membranes were probed with primary antibodies for either GSDMD (G7422, Sigma-Aldrich 1/1000; HPA044487, Sigma-Aldrich, 1/1000), GSDME (ab215191, Abcam, 1/1000), Caspase-1 (AG-20B-0048, Adipogen, 1/1000), Caspase-3 (9661, Cell signaling, 1/1000), Caspase-8 (9746, Cell Signaling, 1/500), IL-1α (500-P21A, PeproTech, 1/500), IL-1β (AF-201-NA, R&D, 1/1000), Article Title: Crystal structure of an adenovirus virus-associated RNA. Article Snippet: Additionally, total PKR was quantified on a separate slot blot using Negative Control:Article Title: nc886 is induced by TGF-β and suppresses the microRNA pathway in ovarian cancer Article Snippet: The information about antibodies is; phospho-PKR (cat# ab32036 from AbCam, Cambridge, MA; diluted to 1/3000), total PKR (cat# sc-707 from Santa Cruz Biotechnology, Dallas, TX; diluted to 1/3000), phospho-eIF2α (cat# 9721S from Cell Signaling Technology, Danvers, MA; diluted to 1/3000), total eIF2α (cat# 9722S from Cell Signaling Technology; diluted to 1/3000), and Article Title: Mechanism of Protein Kinase R Inhibition by Human Cytomegalovirus pTRS1 Article Snippet: Antibodies against the following were used: pTRS1 (1:100 [ 43 ]), His (1:1,000; CST; number 2365S), total Article Title: Crystal structure of an adenovirus virus-associated RNA Article Snippet: Additionally, total PKR was quantified on a separate slot blot using Article Title: Viral sensing by epithelial cells involves PKR- and caspase-3-dependent generation of gasdermin E pores Article Snippet: Total PKR , Article Title: Viral sensing by epithelial cells involves PKR- and caspase-3-dependent generation of gasdermin E pores Article Snippet: Nitrocellulose membranes were probed with primary antibodies for either GSDMD (G7422, Sigma-Aldrich 1/1000; HPA044487, Sigma-Aldrich, 1/1000), GSDME (ab215191, Abcam, 1/1000), Caspase-1 (AG-20B-0048, Adipogen, 1/1000), Caspase-3 (9661, Cell signaling, 1/1000), Caspase-8 (9746, Cell Signaling, 1/500), IL-1α (500-P21A, PeproTech, 1/500), IL-1β (AF-201-NA, R&D, 1/1000), Article Title: Crystal structure of an adenovirus virus-associated RNA. Article Snippet: Additionally, total PKR was quantified on a separate slot blot using Software:Article Title: nc886 is induced by TGF-β and suppresses the microRNA pathway in ovarian cancer Article Snippet: The information about antibodies is; phospho-PKR (cat# ab32036 from AbCam, Cambridge, MA; diluted to 1/3000), total PKR (cat# sc-707 from Santa Cruz Biotechnology, Dallas, TX; diluted to 1/3000), phospho-eIF2α (cat# 9721S from Cell Signaling Technology, Danvers, MA; diluted to 1/3000), total eIF2α (cat# 9722S from Cell Signaling Technology; diluted to 1/3000), and Article Title: Mechanism of Protein Kinase R Inhibition by Human Cytomegalovirus pTRS1 Article Snippet: Antibodies against the following were used: pTRS1 (1:100 [ 43 ]), His (1:1,000; CST; number 2365S), total Article Title: Crystal structure of an adenovirus virus-associated RNA Article Snippet: Additionally, total PKR was quantified on a separate slot blot using Article Title: Viral sensing by epithelial cells involves PKR- and caspase-3-dependent generation of gasdermin E pores Article Snippet: Total PKR , Article Title: Viral sensing by epithelial cells involves PKR- and caspase-3-dependent generation of gasdermin E pores Article Snippet: Nitrocellulose membranes were probed with primary antibodies for either GSDMD (G7422, Sigma-Aldrich 1/1000; HPA044487, Sigma-Aldrich, 1/1000), GSDME (ab215191, Abcam, 1/1000), Caspase-1 (AG-20B-0048, Adipogen, 1/1000), Caspase-3 (9661, Cell signaling, 1/1000), Caspase-8 (9746, Cell Signaling, 1/500), IL-1α (500-P21A, PeproTech, 1/500), IL-1β (AF-201-NA, R&D, 1/1000), Article Title: Crystal structure of an adenovirus virus-associated RNA. Article Snippet: Additionally, total PKR was quantified on a separate slot blot using Infection:Article Title: nc886 is induced by TGF-β and suppresses the microRNA pathway in ovarian cancer Article Snippet: The information about antibodies is; phospho-PKR (cat# ab32036 from AbCam, Cambridge, MA; diluted to 1/3000), total PKR (cat# sc-707 from Santa Cruz Biotechnology, Dallas, TX; diluted to 1/3000), phospho-eIF2α (cat# 9721S from Cell Signaling Technology, Danvers, MA; diluted to 1/3000), total eIF2α (cat# 9722S from Cell Signaling Technology; diluted to 1/3000), and Article Title: Mechanism of Protein Kinase R Inhibition by Human Cytomegalovirus pTRS1 Article Snippet: Antibodies against the following were used: pTRS1 (1:100 [ 43 ]), His (1:1,000; CST; number 2365S), total Article Title: Crystal structure of an adenovirus virus-associated RNA Article Snippet: Additionally, total PKR was quantified on a separate slot blot using Article Title: Viral sensing by epithelial cells involves PKR- and caspase-3-dependent generation of gasdermin E pores Article Snippet: Total PKR , Article Title: Viral sensing by epithelial cells involves PKR- and caspase-3-dependent generation of gasdermin E pores Article Snippet: Nitrocellulose membranes were probed with primary antibodies for either GSDMD (G7422, Sigma-Aldrich 1/1000; HPA044487, Sigma-Aldrich, 1/1000), GSDME (ab215191, Abcam, 1/1000), Caspase-1 (AG-20B-0048, Adipogen, 1/1000), Caspase-3 (9661, Cell signaling, 1/1000), Caspase-8 (9746, Cell Signaling, 1/500), IL-1α (500-P21A, PeproTech, 1/500), IL-1β (AF-201-NA, R&D, 1/1000), Article Title: Crystal structure of an adenovirus virus-associated RNA. Article Snippet: Additionally, total PKR was quantified on a separate slot blot using High Molecular Weight:Article Title: nc886 is induced by TGF-β and suppresses the microRNA pathway in ovarian cancer Article Snippet: The information about antibodies is; phospho-PKR (cat# ab32036 from AbCam, Cambridge, MA; diluted to 1/3000), total PKR (cat# sc-707 from Santa Cruz Biotechnology, Dallas, TX; diluted to 1/3000), phospho-eIF2α (cat# 9721S from Cell Signaling Technology, Danvers, MA; diluted to 1/3000), total eIF2α (cat# 9722S from Cell Signaling Technology; diluted to 1/3000), and Article Title: Mechanism of Protein Kinase R Inhibition by Human Cytomegalovirus pTRS1 Article Snippet: Antibodies against the following were used: pTRS1 (1:100 [ 43 ]), His (1:1,000; CST; number 2365S), total Article Title: Crystal structure of an adenovirus virus-associated RNA Article Snippet: Additionally, total PKR was quantified on a separate slot blot using Article Title: Viral sensing by epithelial cells involves PKR- and caspase-3-dependent generation of gasdermin E pores Article Snippet: Total PKR , Article Title: Viral sensing by epithelial cells involves PKR- and caspase-3-dependent generation of gasdermin E pores Article Snippet: Nitrocellulose membranes were probed with primary antibodies for either GSDMD (G7422, Sigma-Aldrich 1/1000; HPA044487, Sigma-Aldrich, 1/1000), GSDME (ab215191, Abcam, 1/1000), Caspase-1 (AG-20B-0048, Adipogen, 1/1000), Caspase-3 (9661, Cell signaling, 1/1000), Caspase-8 (9746, Cell Signaling, 1/500), IL-1α (500-P21A, PeproTech, 1/500), IL-1β (AF-201-NA, R&D, 1/1000), Article Title: Crystal structure of an adenovirus virus-associated RNA. Article Snippet: Additionally, total PKR was quantified on a separate slot blot using |
